Tshem tawm Cys sab saw tBu Tiv Thaiv Pawg Txheej Txheem

Cys (tBu) Feem ntau hauv TFA thiab I2 yog qhov ruaj khov, yog li lawv feem ntau siv los ua ke los tsim cov peptides nrog peb khub ntawm disulfide bonds thiab saum toj no yam tsis muaj kev cuam tshuam rau yav dhau los disulfide bonds.
Tshem tawm tBu Cov txheej txheem feem ntau suav nrog mercury acetate thiab trichloromethylsilane / Diphenyl sulfoxide txoj kev.
Hauv qab no yog ib qho kev taw qhia rau cov kauj ruam ntawm mercury acetate txoj kev:

1. Yuav coj tBu ntawm peptides yaj hauv dej khov TFA nruab nrab (5-10mg / ml)
2. Ntxiv 10 npaug ntawm qhov sib npaug ntawm mercury acetate thiab do rau 3 teev.
3. Tig nyob rau hauv lub siab txo kom evaporate tshaj TFA, Dissolve nyob rau hauv 10% acetic acid aqueous tov
4. Ntxiv 20 npaug ntawm qhov sib npaug ntawm mercaptoethanol thiab cia nws zaum rau 5 teev
5. Centrifuge tshem tawm precipitate thiab ces desalt los yog HPLC purification.