Shanghai Sonyt Biotechnology Co., Ltd. yog ib qho ntawm cov ua lag luam thiab cov muag khoom ntawm nls (pkkkrkv) (tfa) hauv Suav teb, kuj txhawb kev pabcuam kev cai. Txais tos rau kev lag luam wholesale bulk zoo nls (pkkkrkv) (tfa) los ntawm peb lub hoobkas. Tiv tauj peb kom paub meej ntxiv.
| Kev piav qhia | NLS (PKKKRKV) (TFA) yog 7-amino acid nuclear localization teeb liab peptide nrog cov kab ke Pro-Lys-Lys-Lys-}Arg-Lys-Val (PKKKRKV), muab raws li ntsev acid (PKKKRKV), uas yog ntsev. Cov peptide no sib raug rau cov seem 126-132 ntawm SV40 (Simian Virus 40) Loj T antigen, thiab yog qhov tshawb pom ntxov tshaj plaws thiab tau kawm dav dav tshaj plaws classical monopartite nuclear localization teeb liab (classical monopartite NLS). Xyoo 1984, Daniel Kalderon et al. thawj zaug tau txheeb xyuas qhov PKKKRKV ib ntus raws li lub teeb liab hauv cheeb tsam nuclear los ntawm kev txheeb xyuas kev hloov pauv ntawm SV40 Loj T antigen, kev tshawb pom uas tau pib ua haujlwm ntawm nuclear protein ntshuam mechanism tshawb fawb. Cov yam ntxwv ntawm PKKKRKV ib ntus: (1) tsim los ntawm pawg sib law liag ntawm cov amino acids sib law liag - 3 lysines (Lys, K, txoj haujlwm 127-129) + 1 arginine (Arg, R, txoj hauj lwm 130) {{21} L, txoj hauj lwm tag nrho yooj yim residues, muab cov nqi zog zoo; (2) N-terminal proline (Pro, P, txoj hauj lwm 126) yuav qhia txog kev tig ntawm peptide saw, nthuav tawm cov pawg hauv paus hauv kev pom zoo; (3) C-terminal valine (Val, V, txoj hauj lwm 132) yog ib qho hydrophobic residue uas tuaj yeem koom nrog hydrophobic kev sib cuam tshuam nrog lub hnab ntim khoom xa tuaj. Nuclear ntshuam mechanism ntawm PKKKRKV: (1) qhov loj nuclear localization teeb liab khi qhov chaw (ntau NLS-binding site) ntawm importin tshwj xeeb paub txog cov amino acid pawg hauv PKKKRKV ib ntus, qhov twg Arg130 thiab Lys127/128/129/131 daim ntawv tseem ceeb hydrogen bonds nrog rau cov khoom siv hluav taws xob thiab cov khoom siv hluav taws xob. import; (2) importin tsim ib heterodimer nrog importin, thiab importin mediates cuam tshuam nrog nuclear pore complex (NPC), thauj cov cargo-importin complex los ntawm nuclear pore channel rau hauv lub nucleus; (3) nyob rau hauv lub nucleus, RanGTP khi rau importin, ua rau dissociation ntawm importin / heterodimer thiab tso tawm NLS cargo; (4) importin thiab importin raug thauj rov qab mus rau cytoplasm rau kev rov ua dua tshiab. NLS (PKKKRKV) yog dav siv rau: (1) lub hom phiaj xa khoom thauj xws li cov proteins, peptides, nucleic acids (plasmid DNA, mRNA, siRNA), nanoparticles, thiab cov tshuaj me me rau lub cell nucleus; (2) nuclear ntshuam mechanism kev tshawb fawb, suav nrog importin / lees paub, nuclear pore complex thauj, thiab GTPase Ran txoj cai; (3) txhim kho kev xa tawm nuclear ntawm plasmid DNA, mRNA, thiab CRISPR/Cas9 systems hauv kev kho noob thiab kho noob; (4) kev tsim kho ntawm nuclear kev sojntsuam; (5) raws li ib tug classical NLS cuab tam peptide rau nuclear protein localization kev tshawb fawb thiab nuclear kev loj hlob system; (6) tom qab kev sib txuas nrog DNA, tuaj yeem kho tau zoo DNA hloov pauv hloov pauv thiab txhim kho cov noob hloov pauv tau zoo. Daim ntawv TFA ntsev txhim kho cov dej solubility thiab ruaj khov ntawm peptide, tab sis hauv qee qhov kev sim ntawm tes, TFA yuav tsum tau muab tshem tawm kom tsis txhob muaj peev xwm cytotoxicity. |
Product Basic Information Table
| Khoom Lub Npe Lus Askiv | Product Abbreviation / Alias | Function Tags (comma-sib cais) | Core Structure | Molecular Formula | Exact Mass (Da) | Qhov nruab nrab MW (Da) | Daim ntawv ntsev | Ntev (aa) |
| NLS (PKKKRKV) (TFA) | SV40 tub ntxhais NLS peptide; PKKKRKV NLS peptide TFA ntsev | Nuclear localization teeb liab, kev xa tawm cov protein ntau, kev hloov pauv hloov pauv, kev tsom mus rau nuclear | Core nuclear localization heptapeptide PKKKRKV los ntawm SV40 loj T antigen; lysine/arginine- nplua nuj nyob rau hauv ib theem zuj zus lees paub los ntawm importins ntawm lub nuclear pore complex, mediating active nuclear ntshuam ntawm macromolecules | C₃₉H₇₅N₁₇O₆·xTFA | 829.60 (dawb peptide) | ~ 830.3 (peptide dawb) | Trifluoroacetate (TFA) | 7 |
Physicochemical Solubility & Cia Parameters
| Khoom Code | Qhov tshwm sim | Ntev - Lub Sij Hawm Cia | Luv - Lub Sij Hawm Cia | Kev daws teeb meem | Khoom hmoov ruaj khov |
| PEB-NLS02 | Dawb hmoov | Khaws ntawm -20 degree, kaw, qhuav thiab tiv thaiv los ntawm lub teeb. Aliquot rau cia. | ruaj khov rau 1 lub hlis ntawm 2-8℃nyob rau hauv kaw qhuav; ruaj khov rau shipping ntawm ambient kub. | ruaj khov rau 7 hnub ntawm 4 degree. TFA ntsev yog dawb do soluble nyob rau hauv dej, dilute acetic acid thiab nruab nrab buffers. Kev ruaj ntseg nuclear kev ua haujlwm hauv cheeb tsam hauv alkaline; ntau protonation thiab txo NPC khi nyob rau hauv muaj zog acidic tej yam kev mob. | ruaj khov rau 2 xyoos ntawm -20 degree, qhuav thiab tiv thaiv los ntawm lub teeb. |
Functional Description
| Khoom Code | Frontend Short Summary (rau cov npe tshawb nrhiav) | Cov lus piav qhia txog kev ua haujlwm lom neeg lom neeg | Cov cheeb tsam tshawb fawb (comma- cais) | Lub Hom Phiaj / Txoj Kev |
| PEB-NLS02 | SV40 core NLS peptide PKKKRKV mediates active nuclear ntshuam ntawm macromolecules | PKKKRKV yog qhov tseem ceeb ntawm cov teeb liab hauv zos peptide peptide los ntawm SV40 loj T antigen, feem ntau siv nuclear ntshuam cuab yeej. Cov lysine/arginine- nplua nuj ib theem zuj zus yog tshwj xeeb los ntawm importin / heterodimer ntawm nuclear pore complex, kho kom haum xeeb active nuclear ntshuam ntawm cov proteins, plasmid DNA thiab siRNA, thiab txhim kho kev ua tau zoo ntawm transfection. | Gene transfection & engineering, nuclear protein xa cov kev tshawb fawb, cell biology, nuclear localization mechanism kawm, gene tshuaj txhim kho | Nuclear pore complex (NPC) importin receptor; NLS txoj kev lees paub; active nuclear ntshuam |
Cim npe nrov: nls (pkkkrkv) (tfa), Tuam Tshoj nls (pkkkrkv) (tfa) manufacturers, lwm tus, Hoobkas
